Work

Micropropagation of Black Turmeric (curcuma Caesia Roxb.) Through in Vitro Culture of Rhizome Bud Explants

Year: 2013

Type: article

Abstract: In the present study, preliminary in vitro shoots propagation of Curcuma caesia Roxb. was investigated. Rhizome buds were used as explants and were cultured on Murashige and Skoog (MS) medium containing 6-Benzyl adenine (BA) alone or in combination with α- Naphthalene acetic acid (NAA). The results showed that the optimum shoot proliferation was obtained from MS medium containing 3.0 µM BA + 0.5 µM NAA. In this growth regulator combination, maximum 99.97 % explants produced 10.38 shoots with 4.53 cm length after 8 weeks of culture. Although spontaneous rooting was observed after 4 weeks of cultivation with all treatments using half strength MS medium containing IBA and NAA at different concentrations, high frequency of rooting (89.76 %) was obtained in 3.0 µM IBA (Indole-3-butyric acid) containing medium. The plantlets, thus developed, were hardened and successfully established in natural soil.

Source: Journal of Central European Agriculture

DOI: 10.5513/jcea01/14.3.1289

Authors: Muhammad Shahinozzaman, Molla Ferdous, Muhammad Faruq, Mustafa Azad, Muhammad Amin

Institution: University of Rajshahi

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

Funder: Rajshahi University