Work

Evaluation of Commercially Available Anti–Dengue Virus Immunoglobulin M Tests

Year: 2009

Type: article

Abstract: Anti-dengue virus immunoglobulin M kits were evaluated.Test sensitivities were 21%-99% and specifi cities were 77%-98% compared with reference ELISAs.False-positive results were found for patients with malaria or past dengue infections.Three ELISAs showing strong agreement with reference ELISAs will be included in the World Health Organization Bulk Procurement Scheme.A n estimated 2.5-3 billion persons live in tropical and subtropical regions where dengue virus (DENV) is transmitted (1-3).Absence of inexpensive and accurate tests to diagnose dengue makes case management, surveillance, and outbreak investigation diffi cult.During infection, immunoglobulin (Ig) M against DENV can often be detected ≈5 days after onset of fever (4-6).First-time (primary) DENV infections typically have a stronger and more specifi c IgM response than subsequent (secondary) infections, for which the IgM response is low compared with a strong IgG response.These patterns underscore the need for evaluating the performance of commercially available tests, especially for diagnosis of secondary DENV infections (7-10). The StudyTo provide independent evaluation of dengue diagnostic tests, the United Nations International Children's Emergency Fund/United Nations Development Programme/World Bank/World Health Organization Special Programme for Research and Training in Tropical Diseases and the Pediatric Dengue Vaccine Initiative established a network of 7 laboratories based on criteria related to dengue expertise of the principal investigator, and type, capacity, management of the laboratory.The laboratories contributed serum specimens for the evaluation panel and conducted the evaluation.

Source: Emerging infectious diseases

DOI: 10.3201/eid1503.080923