The Effect of Lanthanides and Actinides on Blood Coagulation. I. Evidence for and Properties of a New Serum Thromboplastic Factor*
Year: 1964
Type: article
Abstract: Thorotrast,' containing 24 to 26% thorium dioxide suspended in a 25% aqueous solution of dextrin and 0.15% methyl parasept (preservative); thorium chloride (ThCl4)2 (analysis: rare earths, 0.03%; Fe, 0.01%; Ag, 0.008%; Ca, 0.01%o; and Mg, 0.01%); uranium chloride (UCl4) ; 3 neodymium nitrate (NdCl3 6H2O) ; 3 cerium chloride (CeCl37H2O); 4 lanthanum chloride (LaCl3- 7H2O) ; 4 uranyl nitrate [UO2(NO3)26H2O)] ; 5 barium sulphate (BaSO4); 5 and inosithin (inositol phospho- lipid)6 were obtained commercially.Oxalated plasma.Nine volumes of human or rabbit blood, obtained by venipuncture with ordinary glass syringe and steel needle, was mixed with 1 vol of 0.10 M sodium oxalate.The blood was centrifuged at 1,500 rpm for 10 minutes and the plasma separated.This oxalated plasma was used fresh or after storage at -120 C.Serum was obtained as above, from blood which was allowed to clot in a glass test tube at room temperature.After 2 hours the clot was gently freed, and the tube was centrifuged at 1,500 rpm for 10 minutes.The separated supernatant serum was used fresh, or after storage at -120 C.Platelets.Nine volumes of blood, obtained as above, was added to 1 vol of 2.5% sodium citrate.The mixture was centrifuged at 800 rpm for 30 minutes, and the separated supernatant plasma was recentrifuged at 4,500 rpm for 30 minutes to sediment the platelets.They were then dispersed and washed 3 times with an equivalent plasma volume of a solution containing 5o% dextrose, 0.2% sodium acetate, and 0.9% sodium chloride.The platelets were subsequently suspended to give a platelet count of 250,000 per mm3 of solution.Platelet-poor plasma.The plasma was separated from the sedimented platelets and aged at 370 C to destroy Factor V (proaccelerin).BaSO4-adsorbed plasma (BaSO4 plasma). 100mg BaSO (chemically pure) 5 was added to each milliliter
DOI: 10.1172/jci104955
Authors: Robert Colman, Benjamin Alexander
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2106856585