Work

Marker Removal System for Thermoanaerobacterium saccharolyticum and Development of a Markerless Ethanologen

Year: 2011

Type: article

Abstract: Marker removal strategies were developed for Thermoanaerobacterium saccharolyticum to select against the pyrF gene and the pta and ack genes. The pta- and ack-based haloacetate selective strategy was subsequently used to create strain M0355, a markerless Δldh Δpta Δack strain that produces ethanol at a high yield.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.01731-10

Authors: A. Joe Shaw, Sean F. Covalla, David A. Hogsett, Christopher D. Herring

Institution: Masco (United States)

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2107012345

FWCI: 2.075

Cites: 14

Cited by: 49

Related to: 10