Fast, Cell‐Compatible Click Chemistry with Copper‐Chelating Azides for Biomolecular Labeling
Year: 2012
Type: article
Abstract: Bring your own copper: Copper-chelating azides undergo much faster click reactions (CuAAC) than nonchelating azides under a variety of biocompatible conditions. This kinetic enhancement allows site-specific protein labeling to be performed on the surface of living cells with only 10–40 μM CuI/CuII (see scheme). Detection sensitivity was also increased for CuAAC detection of alkyne-modified proteins and RNA.
Authors: Chayasith Uttamapinant, Anupong Tangpeerachaikul, Scott Grecian, Scott Clarke, Upinder Singh, Peter Slade, Kyle R. Gee, Alice Y. Ting
Institution: Massachusetts Institute of Technology
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2107344789