Year: 2012
Type: article
Abstract: Two hundred and forty-seven isolates of Acinetobacter baumanii (AB) were identified in the Nashville General Hospital at Meharry epidemiology database for a three year period. Of these isolates, 77 % were multi-drug resis-tant (MRAB). Mechanical ventilation and multiple site recovery were associated with MRAB, and MRAB isolates were associated with increased mortality relative to sensi-tive AB isolates [1]. AB acquires resistance rapidly and the mechanisms are still being identified. Proteomic ana-lysis can identify proteins that change in their expression levels in the presence of antibiotics, a possible mechan-ism of resistance for AB. No proteome databases exist for this organism so antibiotic sensitivity and resistance pro-teomes were generated for MRAB bronchial wash isolate MMC#4. Materials and methods AB MMC#4 was grown in plain LB broth or LB broth supplemented with MIC50 concentrations of levofloxacin, tobramycin, gentamicin, cefotaxime and meropenem. Cell pellets were lysed and total protein run on an SDS-PAGE gel. Protein bands were excised and in-gel digested with trypsin. Resulting peptides were analyzed using a Thermo Finnigan LTQ ion trap mass spectrometer equipped with a 1-D nanoLC pump (Eksigent), Nanospray source (James A Hill Company), and Xcalibur 2.0 SR2 instrument con-trol (Thermo Scientific). Peptides were separated on a
Source: BMC Bioinformatics
Institution: Meharry Medical College
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,990
OpenAlex ID: W2107545048