Work

A Graphene‐Based Platform for the Assay of Duplex‐DNA Unwinding by Helicase

Year: 2010

Type: article

Abstract: Time to unwind: Graphene oxide (GO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. GO selectively binds to unwound fluorescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201001332

Authors: Hongje Jang, Young‐Kwan Kim, Hyun‐Mi Kwon, Woon‐Seok Yeo, Dong‐Eun Kim, Dal‐Hee Min

Institutions: Korea Advanced Institute of Science and Technology, Konkuk University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Research Foundation, National Research Foundation of Korea

OpenAlex ID: W2107569536

FWCI: 10.41

Cites: 41

Cited by: 241

Related to: 10