Work

Submicrometer Pore‐Based Characterization and Quantification of Antibody–Virus Interactions

Year: 2006

Type: article

Abstract: A resistive-pulse sensor (see scheme) employs a submicrometer pore for the detection, characterization, and quantification of the binding of polyclonal antibodies to intact Paramecium bursaria chlorella virus (PBCV-1) particles. The assay is rapid, label-free, requires no immobilization or modification of the antibody or virus, detects the formation of viral aggregates, and can be performed using antibodies in complex media such as serum. The maximum number of antibodies able to bind to the virus was estimated to be 4200±450.

Source: Small

DOI: 10.1002/smll.200600006

Authors: Jeffrey D. Uram, Kevin Ke, Alan J. Hunt, Michael Mayer

Institution: University of Michigan

Language: English

Topic: Nanopore and Nanochannel Transport Studies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, National Institute of General Medical Sciences

Award: Cellular Biotechnology Training Program (CBTP) - Years 31-35