Work

Finding Intermediates in the O2 Activation Pathways of Non-Heme Iron Oxygenases

Year: 2007

Type: review

Abstract: Intermediates in the reaction cycle of an oxygenase are usually very informative with respect to the chemical mechanism of O 2 activation and insertion. However, detection of these intermediates is often complicated by their short lifetime and the regulatory mechanism of the enzyme designed to ensure specificity. Here, the methods used to detect the intermediates in an extradiol dioxygenase, a Rieske cis-dihydrodiol dioxygenase, and soluble methane monooxygenase are discussed. The methods include the use of alternative, chromophoric substrates, mutagenesis of active site catalytic residues, forced changes in substrate binding order, control of reaction rates using regulatory proteins, and initialization of catalysis in crystallo.

Source: Accounts of Chemical Research

DOI: 10.1021/ar700052v

Authors: E. G. Kovaleva, M. B. Neibergall, S. Chakrabarty, J. D. Lipscomb

Institution: University of Minnesota

Language: English

Topic: Metal-Catalyzed Oxygenation Mechanisms

Subfield: Inorganic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: G220149836, OXYGENASE ENZYME MECHANISM, Intermediates in O2 Activation by Oxygenases, Methane Monoxygenase Structure and Function