Work

Rigid DNA Beams for High‐Resolution Single‐Molecule Mechanics

Year: 2013

Type: article

Abstract: Bridging the gap: Rigid DNA linkers (blue, see picture) between microspheres (green) for high-resolution single-molecule mechanical experiments were constructed using DNA origami. The resulting DNA helical bundles greatly reduce the noise generated in studies of conformation changes using optical tweezers and were applied to study small DNA secondary structures.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201302727

Authors: Emanuel Pfitzner, Christian Wachauf, Fabian Kilchherr, Benjamin Pelz, William M. Shih, Matthias Rief, Hendrik Dietz

Institutions: Technical University of Munich, Harvard University, Dana-Farber Cancer Institute, Dana-Farber/Harvard Cancer Center

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Deutsche Forschungsgemeinschaft, Elitenetzwerk Bayern, FP7 Ideas: European Research Council

Awards: G177703080, Single-molecule studies of protein-protein and protein-DNA interactions, enabled by DNA origami

OpenAlex ID: W2107885325

FWCI: 5.224

Cites: 39

Cited by: 119

Related to: 10