Work

Isolation and Applications of Prostate Side Population Cells Based on Dye Cycle Violet Efflux

Year: 2011

Type: article

Abstract: This unit describes methods for digestion of human prostate clinical specimens and dye cycle violet (DCV) staining for identification, isolation, and quantitation of radiolabeled dihydrotestosterone (DHT) retention of side population cells. The principle of the side population assay is based on differential efflux of DCV, a cell-membrane-permeable fluorescent dye, by cells with high ATP-binding cassette (ABC) transporter activity. Cells with high ABC transporter activity that efflux DCV and fall in the lower left quadrant of a flow cytograph are designated as "side population" cells. This unit emphasizes tissue digestion, DCV staining, flow settings for sorting side population cells, and quantitation of radiolabeled DHT retention.

Source: Current Protocols in Toxicology

DOI: 10.1002/0471140856.tx2202s47

Authors: Kalyan J. Gangavarapu, Wendy J. Huss

Institution: Roswell Park Comprehensive Cancer Center

Language: English

Topic: Prostate Cancer Treatment and Research

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases