Year: 1971
Type: article
Abstract: Summary. The preparation of morphologically distinct monolayers of cells by tissue culture of dissected seminiferous tubules and of the intertubular fraction of the adult rat testis is reported. The cells comprising the monolayers obtained by tissue culture of the intertubular fraction of the testis represent interstitial cells, identification being based on light microscopy, 3-β hydroxysteroid dehydrogenase histochemistry, electron microscopy and the ability of the cells to localize 125I-labelled lh. The cell monolayers obtained from the dissected seminiferous tubules arise from the immediate peritubular tissue consisting of myoid cells and lymphatic endothelium. The cells show weak histochemical reactions for 3-β hydroxysteroid dehydrogenase activity and ultrastructural features of myoid cells and fibroblasts. No localization of either 125I-labelled fsh or lh could be demonstrated in these cells by autoradiography.
Source: Reproduction
Authors: D. M. de KRETSER, K. J. CATT, M. L. DUFAU, B. HUDSON
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2108224348