The identification of unique serum proteins of HIV-1 latently infected long-term non-progressor patients
Year: 2010
Type: article
Abstract: BACKGROUND: The search for disease biomarkers within human peripheral fluids has become a favorable approach to preventative therapeutics throughout the past few years. The comparison of normal versus disease states can identify an overexpression or a suppression of critical proteins where illness has directly altered a patient's cellular homeostasis. In particular, the analysis of HIV-1 infected serum is an attractive medium with which to identify altered protein expression due to the ease and non-invasive methods of collecting samples as well as the corresponding insight into the in vivo interaction of the virus with infected cells/tissue. The utilization of proteomic techniques to globally identify differentially expressed serum proteins in response to HIV-1 infection is a significant undertaking that is complicated due to the innate protein profile of human serum. RESULTS: Here, the depletion of 12 of the most abundant serum proteins, followed by two-dimensional gel electrophoresis coupled with identification of these proteins using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, has allowed for the identification of differentially expressed, low abundant serum proteins. We have analyzed and compared serum samples from HIV-1 infected subjects who are being treated using highly active antiretroviral therapy (HAART) to those who are latently infected but have not progressed to AIDS despite the absence of treatment, i.e. long term non-progressors (LTNPs). Here we have identified unique serum proteins that are differentially expressed in LTNP HIV-1 patients and may contribute to the ability of these patients to combat HIV-1 infection in the absence of HAART. We focused on the cdk4/6 cell cycle inhibitor p16INK4A and found that the treatment of HIV-1 latently infected cell lines with p16INK4A decreases viral production despite it not being expressed endogenously in these cells. CONCLUSIONS: Identification of these unique proteins may serve as an indication of altered viral states in response to infection as well as a natural phenotypic variability in response to HIV-1 infection in a given population.
Source: AIDS Research and Therapy
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,990
Funders: George Washington University, Academia Sinica, National Institutes of Health, University of California, San Francisco, National Institute on Drug Abuse, National Cancer Institute, National Institute on Deafness and Other Communication Disorders, National Institute of Allergy and Infectious Diseases, National Institute of Child Health and Human Development, National Center for Research Resources, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: G1252706584, G1383752764, G1423343497, G1440661678, HIV-1 TAR derived miRNA: Implications for Latency and Pathogenesis, Clinical and Translational Science Institute, G1653728403, G1689765846, G1919789535, G2037985070, G2210861095, G2436660153, G2567544100, G2574405597, G2674972404, G2692709432, G2889922178, G3009646583, G3079612072, G3079921036, G316338625, G3212103026, G3222910550, G3264576717, G3322721267, The Connie Wofsy Women's HIV Study, Brooklyn Women's Interagency HIV Study (WIHS) V, G3658850503, G3901006228, G398667773, G4097647764, G4210511483, G4419450409, WIHS Data Management and Analysis Center (WDMAC), G4547062945, G4622231416, Southern California Women's Interagency HIV Study Consortium, G4664570284, G4804712267, G4897434353, HIV-1 Inhibition Using Tat peptide derivatives, G499585988, Chicago WIHS Consortium V (CWC), G5182092287, G5282551706, G5514441876, Washington Women's Interagency HIV/AIDS Study, G6295786800, G6694807131, G6797327535, Effect of chromatin remodelers/modifiers on HIV-1 Tat activated transcription, G7195401492, G758528517, Women's Interagency HIV Study (WIHS-V), G8121648955, G826976987, G8350406629, G8355300055, G893076474, G8962527242, G8964979204, G9000034972, G9000058261, G9000073987, G9000237332, G9000338758, G90347297, G920507375, G925273713, G937853209, G976727283
OpenAlex ID: W2108441567