Work

Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays

Year: 2009

Type: article

Abstract: Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a "chemical nose/tongue" approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.0900975106

Authors: Avinash Bajaj, Oscar R. Miranda, Ik-Bum Kim, Ronnie L. Phillips, D. Joseph Jerry, Uwe H. F. Bunz, Vincent M. Rotello

Institutions: University of Massachusetts Amherst, University of Wisconsin–Madison, Georgia Institute of Technology

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Science Foundation, U.S. Department of Energy, Center for Hierarchical Manufacturing, National Science Foundation, National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases

Awards: Detection of Bacteria using Nanoparticle-Polymer Sensors, NSEC: Center for Hierarchical Manufacturing, G222768804, SUGAR SUBSTITUTED POLY (PARAPHENYLENEETHYNLENE) POLYMERS AT THE INTERFACE OF BIOMOLECULAR MATERIALS AND SEMICONDUCTORS, Hormonal regulation of p53 activity in mammary tissue, Genetic modifiers of mammary tumor susceptibility, Rapid Multi-Channel Serum Profiling for Cardiac Disease using Fluorescent Nanosensors, G6833135833

OpenAlex ID: W2108464046

FWCI: 8.688

Cites: 44

Cited by: 314

Related to: 10