Work

Use of a High-Resolution Melt Assay To Characterize Codon 54 of the cyp51A Gene of Aspergillus fumigatus on a Rotor-Gene 6000 Instrument

Year: 2010

Type: article

Abstract: A high-resolution melt (HRM) assay using a Rotor-Gene 6000 instrument was developed to characterize the codon for glycine 54 in the cyp51A genes from 13 reference isolates and 12 clinical isolates of Aspergillus fumigatus. Mutations in this codon confer reduced susceptibility to itraconazole and posaconazole. The assay is simple to perform, and a result of "wild type" or "mutant" is available after approximately 1 h following DNA extraction using commercially available reagents and conventional primers.

Source: Antimicrobial Agents and Chemotherapy

DOI: 10.1128/aac.01712-09

Authors: M. J. Tuohy, V. Reja, S. Park, D. S. Perlin, M. Wnek, G. W. Procop, B. Yen-Lieberman

Institutions: Cleveland Clinic, Rutgers New Jersey Medical School

Language: English

Topic: Antifungal resistance and susceptibility

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2108567523

FWCI: 0.383

Cites: 33

Cited by: 10

Related to: 10