Work

Quantitative Analysis of Copy Number Variants Based on Real‐Time LightCycler PCR

Year: 2014

Type: article

Abstract: Quantitative real‐time PCR is PCR visualized in real time by the use of fluorescent or intercalating dyes, which are employed to measure gene expression or gene quantification including contiguous gene deletions or duplications. A simple method is described here to quantify DNA copy number from human samples. Curr. Protoc. Hum. Genet. 80:7.21.1‐7.21.8. © 2014 by John Wiley & Sons, Inc.

Source: Current Protocols in Human Genetics

DOI: 10.1002/0471142905.hg0721s80

Authors: Lijiang Ma, Wendy K. Chung

Institution: Columbia University

Language: English

Topic: Genomic variations and chromosomal abnormalities

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Heart, Lung, and Blood Institute

Award: Molecular approaches to gene identification in congenital heart disease