Work

Fine-scale mapping inNeurospora crassaby using genome-wide knockout strains

Year: 2012

Type: article

Abstract: Fine-scale genetic mapping is often hindered by the lack of adequate markers surrounding the locus of interest. In the filamentous ascomycete Neurospora crassa, the genome has been sequenced and an effort has been made to generate genome-wide deletion strains for the entire gene set. Accordingly, the hygromycin-resistant marker in each deletion strain can be used as a mapping locus in a classical three-point cross, along with the mapping target and a standard marker. We have demonstrated the feasibility of this fine-scale mapping approach in N. crassa by refining the location of r(Sk-2).

Source: Mycologia

DOI: 10.3852/11-062

Authors: Thomas M. Hammond, David G. Rehard, Bryant C. Harris, Patrick K.T. Shiu

Institution: University of Missouri

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, University of Missouri, National Institute of General Medical Sciences

Award: Genetic and Molecular Dissection of Meiotic Silencing by Unpaired DNA