Work

Enhancement of the polycation‐mediated DNA uptake and cell transfection with Pluronic P85 block copolymer

Year: 1996

Type: article

Abstract: Polyelectrolyte complexes formed between DNA and poly(N-ethyl4-vinylpyridinium) cations were shown to effectively transfect mammalian cells [7]. This work suggests that the polycation-mediated uptake of the plasmid DNA and cell transfection are significantly enhanced when these complexes are administered simultaneously with a poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) copolymer, Pluronic P85. The uptake studies were performed using radioactively labeled pRSV CAT plasmid on NIH 3T3, MDCK, and Jurkat cell lines. The transfection was investigated by chloramphenicol acetyltransferase assay using 3T3 cells as a model. The effects reported may be useful for the enhancement of the polycation-mediated cell transfection.

Source: FEBS Letters

DOI: 10.1016/0014-5793(96)00607-2

Authors: Irina Astafieva, Irina Maksimova, Eugenii Lukanidin, Valery Alakhov, Alexander Kabanov

Institutions: Lomonosov Moscow State University, Institute of Gene Biology

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Lomonosov Moscow State University

OpenAlex ID: W2109247464

FWCI: 0.371

Cites: 11

Cited by: 66

Related to: 10