Single Reporter for Targeted Multimodal in Vivo Imaging
Year: 2012
Type: article
Abstract: We have developed a multifaceted, highly specific reporter for multimodal in vivo imaging and applied it for detection of brain tumors. A metabolically biotinylated, membrane-bound form of Gaussia luciferase was synthesized, termed mbGluc-biotin. We engineered glioma cells to express this reporter and showed that brain tumor formation can be temporally imaged by bioluminescence following systemic administration of coelenterazine. Brain tumors expressing this reporter had high sensitivity for detection by magnetic resonance and fluorescence tomographic imaging upon injection of streptavidin conjugated to magnetic nanoparticles or fluorophore, respectively. Moreover, single photon emission computed tomography showed enhanced imaging of these tumors upon injection with streptavidin complexed to (111)In-DTPA-biotin. This work shows for the first time a single small reporter (∼40 kDa) which can be monitored with most available molecular imaging modalities and can be extended for single cell imaging using intravital microscopy, allowing real-time tracking of any cell expressing it in vivo.
DOI: 10.1021/ja209868g
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: G1049334674, Center for Molecular Imaging Research at MGH/HMS, G2877435986, Imaging and Treating Inflammation in Stroke by Targeting Myeloperoxidase, Myeloperoxidase, imaging biomarker and treatment target for multiple sclerosis, Molecular imaging of vascular pathology, Imaging and therapy of tumors using biotinylated cell surface docking sites, Interdepartmental Neuroscience Center, G901775365
OpenAlex ID: W2109642767