In the paper "Human Dis3p, Which Binds to Either GTP- or GDP-Ran, Complements Saccharomyces cerevisiae dis3"
Year: 1998
Type: article
Abstract: Fig. 4A and Fig. 5A on page 887 and 888, respectively, were inadvertently interchanged and incorrectly printed. The correct figures and corresponding legends are shown below. Fig. 4. S. cerevisiae Dis3p is localized in the nucleolus. A: Characterization of the anti-Dis3sc-antibodies. Crude extracts of S. cerevisiae strains 37C19 {Δdis3 [YCpDIS3sc]} (lane 1), 37C19-A {Δdis3 [pGAP-GSTDIS3sc]} (lane 2), and YPH501 (lane 3) were prepared by vortexing with glass beads, and then subjected to 7.5% SDS-PAGE, transferred to PVDF membrane and immunoblotted with the anti-Dis3sc antibodies, as described (13). Fig. 5. Dis3p binds to both GDP and GTP-Ran. A: SDS-polyacrylamide gel electro-phoretic analysis of purified GST-fused human Dis3p. About 80 μg of GST-fused human Dis3p was digested with thrombin and then the beads were spun down. GST-fused human Dis3p, and proteins in the supernatant and on the beads were electrophoresed on SDS-polyacrylamide (10%) gels and then stained with Coomassie Brilliant Blue. Lane 1: GST-human Dis3p. Lane 2: human Dis3p digested with thrombin. Lane 3: GST-beads.
Source: The Journal of Biochemistry
Authors: T. Shiomi, K. Fukushima, N. Suzuki, N. Nakashima, E. Noguchi, T. Nishimoto
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2109644053