Work

Microarray Analysis of Transposon Insertion Mutations inBacillus anthracis: Global Identification of Genes Required for Sporulation and Germination

Year: 2007

Type: article

Abstract: A transposon site hybridization (TraSH) assay was developed for functional analysis of the Bacillus anthracis genome using a mini-Tn10 transposon which permitted analysis of 82% of this pathogen's genes. The system, used to identify genes required for generation of infectious anthrax spores, spore germination, and optimal growth on rich medium, was predictive of the contributions of two conserved hypothetical genes for the phenotypes examined.

Source: Journal of Bacteriology

DOI: 10.1128/jb.01860-06

Authors: William A. Day, Suzanne L. Rasmussen, Beth M. Carpenter, Scott N. Peterson, Arthur M. Friedlander

Institution: United States Army Medical Research Institute of Infectious Diseases

Language: English

Topic: Bacillus and Francisella bacterial research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, Defense Threat Reduction Agency, National Institute of Allergy and Infectious Diseases

Awards: G1795832447, Pathogen Functional Genomics Resource Center , G4393179445