Work

Using genomics and proteomics to investigate mechanisms of transcriptional silencing in Saccharomyces cerevisiae

Year: 2006

Type: review

Abstract: Silent chromatin in budding yeast is characterized by the presence of a specialized chromatin modification complex consisting of silent information regulator (Sir) proteins, closely packed pairs of nucleosomes, and hypoacetylated and hypomethylated histones. How this specialized chromatin is established, maintained and inherited has been extensively studied. Less investigated are the determinants that constrain its linear spread along the chromatin fibre and the manner by which it represses gene transcription. Here we review the essential features of SIR-mediated heterochromatin, and discuss genomic and proteomic approaches for discerning the composition of its boundaries and for elucidating the mechanisms by which it silences transcription.

Source: Briefings in Functional Genomics and Proteomics

DOI: 10.1093/bfgp/ell035

Authors: L. Gao, D. S. Gross

Institutions: Louisiana State University Health Sciences Center Shreveport, Louisiana State University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Science Foundation

Award: Silenced Chromatin: Mechanisms of Transcriptional Repression