Work

The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney

Year: 2005

Type: article

Abstract: Mutation of the gene encoding the Mediator component thyroid hormone receptor-associated protein (TRAP)230/MED12 affects the development of multiple systems in zebrafish embryogenesis. We isolated two ethylnitrosourea-induced alleles in the gene encoding this protein and named the locus kohtalo (kto) after the homologous locus in Drosophila. Homozygous kto mutant zebrafish embryos show defects in brain, neural crest, and kidney development and die at approximately 6 days postfertilization. In the affected tissues, differentiation is initiated and many cell type-specific genes are expressed, but there is a failure of morphogenesis and failure to complete differentiation. These results suggest that critical targets of TRAP230 function may include proteins important for cell mobility, cell sorting, and tissue assembly.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.0509457102

Authors: Sung-Kook Hong, Caroline E. Haldin, Nathan D. Lawson, Brant M. Weinstein, Igor B. Dawid, Neil A. Hukriede

Institutions: National Institutes of Health, University of Pittsburgh, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Language: English

Topic: Developmental Biology and Gene Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

OpenAlex ID: W2110151622

FWCI: 2.636

Cites: 50

Cited by: 81

Related to: 10