puckered encodes a phosphatase that mediates a feedback loop regulating JNK activity during dorsal closure in Drosophila
Year: 1998
Type: preprint
Abstract: The activation of MAPKs is controlled by the balance between MAPK kinase and MAPK phosphatase activities. The latter is mediated by a subset of phosphatases with dual specificity (VH-1 family). Here, we describe a new member of this family encoded by the puckered gene of Drosophila. Mutations in this gene lead to cytoskeletal defects that result in a failure in dorsal closure related to those associated with mutations in basket, the Drosophila JNK homolog. We show that puckered mutations result in the hyperactivation of DJNK, and that overexpression of puc mimics basket mutant phenotypes. We also show that puckered expression is itself a consequence of the activity of the JNK pathway and that during dorsal closure, JNK signaling has a dual role: to activate an effector, encoded by decapentaplegic, and an element of negative feedback regulation encoded by puckered.
Source: Genes & Development
DOI: 10.1101/gad.12.4.557
Authors: E. Martin-Blanco, A. Gampel, J. Ring, K. Virdee, N. Kirov, A. M. Tolkovsky, A. Martinez-Arias
Institutions: University of Cambridge, New York University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Funders: Wellcome Trust, European Commission
OpenAlex ID: W2110367574