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Effect of Growth Phase Feeding Strategies on Succinate Production by Metabolically Engineered Escherichia coli

Year: 2009

Type: article

Abstract: Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt showed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.02190-09

Authors: Min Jiang, Shu-wen Liu, Jiang-feng Ma, Ke-quan Chen, Li Yu, Fang-fang Yue, Bing Xu, Ping Wei

Institutions: Nanjing Tech University, State Key Laboratory of Materials-Oriented Chemical Engineering

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: green

OpenAlex ID: W2110534321

FWCI: 1.209

Cites: 23

Cited by: 63

Related to: 10