Work

Time‐Controlled Microfluidic Seeding in nL‐Volume Droplets To Separate Nucleation and Growth Stages of Protein Crystallization

Year: 2006

Type: article

Abstract: Sowing seeds: The growth of well-ordered protein crystals requires control of the two key stages of crystallization, namely, nucleation and growth. However, the ideal conditions for these two stages are often different. A microfluidic system (see picture) was used to perform time-controlled crystal seeding in nL volumes. Single crystals of the protein Oligoendopeptidase F were obtained and used to determine its X-ray crystal structure.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.200602946

Authors: Cory J. Gerdts, Valentina Tereshko, Maneesh K. Yadav, Irina Dementieva, Frank Collart, Andrzej Joachimiak, Raymond C. Stevens, Peter Kuhn, Anthony Kossiakoff, Rustem F. Ismagilov

Institutions: University of Chicago, Scripps Research Institute, Argonne National Laboratory

Language: English

Topic: Crystallization and Solubility Studies

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering, National Center for Research Resources

Awards: G2363639003, G3248058419, G3250212683, G402281873, G4036938481, BioCARS: A Synchroton Structural Biology Resource, Gene to Structure for $10K (GTS10K), New Microfluidic Technology as Research Tool for Biology

OpenAlex ID: W2111311631

FWCI: 7.418

Cites: 46

Cited by: 124

Related to: 10