Year: 2014
Type: review
Abstract: Eukaryotic and prokaryotic organisms possess huge numbers of uncharacterized enzymes. Selective inhibitors offer powerful probes for assigning functions to enzymes in native biological systems. Here, we discuss how the chemical proteomic platform activity-based protein profiling (ABPP) can be implemented to discover selective and in vivo-active inhibitors for enzymes. We further describe how these inhibitors have been used to delineate the biochemical and cellular functions of enzymes, leading to the discovery of metabolic and signaling pathways that make important contributions to human physiology and disease. These studies demonstrate the value of selective chemical probes as drivers of biological inquiry.
Source: Annual Review of Biochemistry
Authors: Micah J. Niphakis, Benjamin F. Cravatt
Institution: Scripps Research Institute
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
Awards: G120164892, Chemical probes to assess endocannabinoid localization, storage, and transport, G2418409658, The Comprehensive Center for Chemical Probe Discovery and Optimization at Scripps, Massively Parallel Identification of Protein Ligands, G3510558551, G3841156357, G4321753651, Monoacylglycerol Lipase Inhibitors for Treating Opioid Use Disorders, CHEMOPROTEOMIC METHODS FOR SERINE HYDROLASE INHIBITOR DEVELOPMENT, G6296143508, G6838120957, G813069037, G9000078377, G9000088016, G9000153503, G9000272408, G9000277678
OpenAlex ID: W2111385909