Rol del óxido nítrico en modelos experimentales propuestos para inducir inflamación gástrica e intestinal en ratas
Year: 2008
Type: dissertation
Abstract: --- Nowadays it comes using several models who use etanol (EtOH) and indomethacin (Indo) to induce the gastric and intestinal inflammation in experimental form. In order to determine which is the role of nitric oxide (NO) in the gastric and intestinal inflammation, we have used sodium nitrite (NIT), sodium nitroprusiate (NPS) and metronidazole (MET) as NO donors; NG-nitro-L-argininemethyl ester L-NAME) was used as unspecific NO syntasa inhibitor and methylene blue (AM) like guanidine cyclical monophosphate (GMPc) inhibitor. 620 albino male rats (Holtzman) were used who before were conditioned in the laboratory of Pharmacology, Toxicology of the UNMSM Faculty of Veterinary Medicine and private of food but not of water for 24 hours before the induction of the acute inflammation induced for EtOH (Robert et al., 1979), and with Indo (Davies and Jamali, 1997). The intestinal acute inflammation (Ramos Anicama, 2003) and the intestinal chronic inflammation (Yamada et al., 1993) was induced by Indo. The macroscopic damage was determined in a calibrated microscope and also by computerized planimetry. NO determination was quantifying indirectly in the plasma its stable metabolite: the nitrite, by means of Griess reaction. Our results demonstrate that NO take part as cytoprotector in the models of acute inflammation; and is proinflammatory in the intestinal chronic inflammation model. The discovery that NO take part in the model of Yamada et al. has allowed us to find a new scientific evidence of the inflammatory activity of the leaves of the Uncaria tomentosa Willd D.C. (cat´s claw).
Author: Pedro Angulo Herrera
Language: Spanish
Subfield: Pharmacology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2111565521