Work

Protein induced fluorescence enhancement (PIFE) for probing protein–nucleic acid interactions

Year: 2013

Type: review

Abstract: Single molecule studies of protein-nucleic acid interactions shed light on molecular mechanisms and kinetics involved in protein binding, translocation, and unwinding of DNA and RNA substrates. In this review, we provide an overview of a single molecule fluorescence method, termed "protein induced fluorescence enhancement" (PIFE). Unlike FRET where two dyes are required, PIFE employs a single dye attached to DNA or RNA to which an unlabeled protein is applied. We discuss both ensemble and single molecule studies in which PIFE was utilized.

Source: Chemical Society Reviews

DOI: 10.1039/c3cs60201j

Authors: Helen Hwang, Sua Myong

Institutions: University of Illinois Urbana-Champaign, Carl R. Woese Institute for Genomic Biology

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Award: G3374670784