Work

A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

Year: 2013

Type: article

Abstract: The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

Source: Chemical Communications

DOI: 10.1039/c3cc44783a

Authors: Abhinav P. Acharya, Parsa M. Nafisi, Austin Gardner, Joanna L. MacKay, Kousik Kundu, Sanjay Kumar, Niren Murthy

Institutions: University of California System, University of California, Berkeley, LI-COR Biosciences (United States)

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases

Awards: Cellular mechanobiology: Biophysics and therapeutics, Plaque detection with the hydrocyanines, Fundamental Mechanobiology of Tumor Progression, G7640183395, Maltodextrin based antibiotics, UCSF/UCB Joint Graduate Group in Bioengineering

OpenAlex ID: W2111998859

FWCI: 0.599

Cites: 21

Cited by: 33

Related to: 10