Construction of a High Efficiency PCR Products Cloning T Vector Using pGEM-5zf (+).
Year: 2009
Type: article
Abstract: A highly efficient cloning vector was constructed for cloning PCR products by inserting an 80 bp DNA fragment into pGEM-5zf (+) vector. The Xcm I digestion of this vector gave rise to a 3' overhanging deoxythymidine offering the possibility of cloning PCR products with 3' adenosine overhang created by Taq DNA polymerase. Furthermore, two EcoR I sites were added to the construct for identification of recombinant plasmids using a single restriction enzyme. Taken together, the more efficient cloning performance and the lower cost of this vector as compared to the commercial T vector, suggests that it may be one of the best T vectors for cloning of PCR products.
Source: Europe PMC (PubMed Central)
Authors: Yaofeng Zhao, Zhancai Liu, Shuyang Yu, Sicheng Wen, Lennart Hammarstrom, Hodjattallah Rabbani
Institution: Karolinska University Hospital
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2112016469