Work

Condensins Promote Coorientation of Sister Chromatids During Meiosis I in Budding Yeast

Year: 2010

Type: article

Abstract: The condensin complex is a key determinant of higher-ordered chromosome structure. We show here that the complex is also important for the correct alignment of chromosomes on the meiosis I spindle. Unlike during mitosis and meiosis II, when sister chromatids attach to microtubules emanating from opposite spindle poles (biorientation), accurate meiosis I chromosome segregation requires that sister chromatids attach to microtubules emanating from the same spindle pole (co-orientation). The monopolin complex, consisting of Lrs4, Csm1, and the meiosis-specific component Mam1, brings about meiosis I co-orientation. We find that in the absence of functional condensin complexes, a fraction of sister kinetochores biorient on the meiosis I spindle and association of the monopolin complex subunit Mam1 with kinetochores is decreased. Our studies uncover a new locus-specific effect of the condensin complex.

Source: Genetics

DOI: 10.1534/genetics.110.115139

Authors: Ilana L Brito, Hong-Guo Yu, Angelika Amon

Institutions: Howard Hughes Medical Institute, Koch Institute for Integrative Cancer Research At MIT, Massachusetts Institute of Technology, Florida State University

Language: English

Topic: Microtubule and mitosis dynamics

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Howard Hughes Medical Institute, National Institute of General Medical Sciences

Awards: G3255744735, G872090586