Work

A genetically encoded aldehyde for rapid protein labelling

Year: 2014

Type: article

Abstract: Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.

Source: Chemical Communications

DOI: 10.1039/c4cc02000f

Authors: Alfred Tuley, Yan-Jiun Lee, Bo Wu, Zhiyong U. Wang, Wenshe R. Liu

Institutions: Texas A&M University, Troy University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Award: Phage Display with Two Genetically Incorporated Noncanonical Amino Acids