Work

Climbing the vertebrate branch of U1A/U2B″ protein evolution

Year: 2014

Type: article

Abstract: In the vertebrate lineage of the U1A/U2B″/SNF protein family, the U1A and U2B″ proteins bind to RNA stem-loops in the U1 or U2 snRNPs, respectively. However, their specialization is fairly recent, as they evolved from a single ancestral protein. The progress of their specialization (subfunctionalization) can be monitored by the amino acid sequence changes that give rise to their modern RNA-binding specificity. Using ancestral sequence reconstruction to predict the intermediates on the evolutionary branch, a probable path of sequential changes is defined for U1A and U2B″. The RNA-binding affinity for U1A/U2B″ protein ancestors was measured using modern U1 and U2 snRNA stem-loops and RNA stem-loop variants to understand how the proteins' RNA specificities evolved.

Source: RNA

DOI: 10.1261/rna.044255.114

Authors: Kimberly J. Delaney, Sandra G. Williams, Mariah Lawler, Kathleen B. Hall

Institution: Washington University in St. Louis

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Coevolution of snrnp U1A/U2B proteins and snRNA stemloops, The contribution of protein conformational instability to RNA binding