Work

CaRuby-Nano: a novel high affinity calcium probe for dual color imaging

Year: 2015

Type: article

Abstract: The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.

Source: eLife

DOI: 10.7554/elife.05808

Authors: Mayeul Collot, Christian D Wilms, Asma Bentkhayet, Païkan Marcaggi, Kiri Couchman, Serge Charpak, Stéphane Dieudonné, Michael Häusser, Anne Feltz, Jean-Maurice Mallet

Institutions: École Normale Supérieure - PSL, Laboratoire des Biomolécules, Université Pierre-et-Marie-Curie, University College London, Institut de Biologie de l'École Normale Supérieure, Inserm, Neurophysiologie et nouvelles microscopies, Université Paris Descartes

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,500

Funders: Wellcome Trust, Gatsby Charitable Foundation, Human Frontier Science Program, Agence Nationale de la Recherche, Fondation pour la Recherche Médicale, Fédération pour la Recherche sur le Cerveau, European Research Council

Awards: The elements of cerebellar computation., INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE, G2079521050, G3656467104, G4039092873, G4704980588, G474771382, G4879523425, G6761010743, G8461320036, G9000280678

OpenAlex ID: W2113512934

FWCI: 4.031

Cites: 38

Cited by: 30

Related to: 10