Regulation of prostanoid effects in whole blood : immediate-early anti-inflammatory effects of prostaglandin E2
Year: 2008
Type: dissertation
Abstract: To understand the regulation of the pathophysiological processes, such as inflammation, thrombosis and atherosclerosis, it is very important to characterize the interactions between circulating cells and which molecules that contributes to and promote these interactions. As a part of this, the role of eicosanoids in cell-cell interactions in whole blood ex vivo and isolated blood cells are investigated. In this study, various inhibitors were used to regulate the amount of prostaglandins (and leukotrienes) and how these eicosanoids affect the activity- and expression level of tissue factor (TF), cytokines, enzymes, and receptors involved in the intercellular communication. Before the inhibition study, time course experiments revealed that the incubation times between 1.5 h and 2 h was sufficient for further study of the parameters under investigation. \nIn the inhibition study, whole blood samples were preincubated with different eicosanoid inhibitors, and then stimulated with LPS for ninety minutes prior to TF activity measurement and real-time PCR analysis of cytokine gene expressions. Aspirin did not significantly enhance the lipopolysaccharide (LPS)-induced TF activity in whole blood, however a trend for enhance induction was indicated.
Author: Danh Phung
Language: English
Subfield: Pharmacology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2114079866