Work

Critical evaluation of membrane supports for use in quantitative hybridizations

Year: 1996

Type: article

Abstract: The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher & Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of detectability provided by the Magna Charge and Immobilon-N membranes were 20 to 50 times better than those obtained with the MagnaGraph, Magna, and Nytran membranes. The variability of the signal response for individual membranes ranged from 10 to 50%, with the Magna Charge and Immobilon-N membranes demonstrating the lowest variability.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.62.1.300-303.1996

Authors: L Raskin, W C Capman, M D Kane, B E Rittmann, D A Stahl

Institutions: University of Illinois Urbana-Champaign, Augsburg University, Smithsonian Institution, Northwestern University

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2114280024

FWCI: 0.632

Cites: 8

Cited by: 21

Related to: 10