Work

Activation of topoisomerase II-mediated excision of chromosomal DNA loops during oxidative stress

Year: 1999

Type: preprint

Abstract: Hydrogen peroxide (H2O2), a reactive oxygen species (ROS), is known to induce oxidative stress and apoptosis. U937 cells treated with H2O2 were shown to produce high molecular weight (HMW) DNA fragments approximately 50 to 100 kb in size in <1 min. The formation of these HMW DNA fragments is reversible and shown to be mediated by DNA topoisomerase II (TOP2). Following this initial event, formation of irreversible HMW DNA fragments and nucleosomal ladders occurs. Our results thus demonstrate a potential role of TOP2 in oxidative damage of DNA and apoptotic cell death.

Source: Genes & Development

DOI: 10.1101/gad.13.12.1553

Authors: T.-K. Li, A. Y. Chen, C. Yu, Y. Mao, H. Wang, L. F. Liu

Institutions: University of Medicine and Dentistry of New Jersey, Rutgers, The State University of New Jersey

Language: English

Topic: Cancer therapeutics and mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences

Awards: G2450052837, FUNCTIONAL AND MECHANISTIC STUDIES OF DNA TOPOISOMERASES