Year: 2015
Type: article
Abstract: Fluorocarbons are lipophobic and non-polar molecules that exhibit remarkable biocompatibility, with applications in liquid ventilation and synthetic blood. The unique properties of these compounds have also enabled mass spectrometry imaging of tissues where the fluorocarbons act as a Teflon-like coating for nanostructured surfaces to assist in desorption/ionization. Here we report fluorinated gold nanoparticles (f-AuNPs) designed to facilitate nanostructure imaging mass spectrometry. Irradiation of f-AuNPs results in the release of the fluorocarbon ligands providing a driving force for analyte desorption. The f-AuNPs allow for the mass spectrometry analysis of both lipophilic and polar (central carbon) metabolites. An important property of AuNPs is that they also act as contrast agents for X-ray microtomography and electron microscopy, a feature we have exploited by infusing f-AuNPs into tissue via fluorocarbon liquids to facilitate multimodal (molecular and anatomical) imaging. Perfluorinated organic molecules have shown many uses, including as imaging agents. Here, the authors report that fluorinated gold nanoparticles offer an effective means of mass spectrometry tissue imaging, in addition to facilitating X-ray analysis providing complementary information to mass spectral images.
Source: Nature Communications
DOI: 10.1038/ncomms6998
Authors: Michael E. Kurczy, Zheng-Jiang Zhu, Julijana Ivanisevic, Adam M. Schuyler, Kush Lalwani, Antonio F. Santidrian, John M. David, Anand Giddabasappa, Amanda J. Roberts, Hernando J. Olivos, Peter J. O’Brien, Lauren Franco, Matthew W. Fields, Liliana P. Paris, Martin Friedlander, Caroline H. Johnson, Adrian A. Epstein, Howard E. Gendelman, Malcolm R. Wood, Brunhilde H. Felding, Gary J. Patti, Mary E. Spilker, Gary Siuzdak
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $6,990
Funders: U.S. Department of Defense, U.S. Department of Energy, California Institute of Regenerative Medicine, National Institutes of Health, Office of Science, National Institute on Aging, National Heart, Lung, and Blood Institute, National Institute of Mental Health, National Institute on Drug Abuse, National Eye Institute, National Cancer Institute, National Institute of Neurological Disorders and Stroke, Lawrence Berkeley National Laboratory
Awards: G1079818582, G1143651532, G1286236842, G1307824525, NanoART Manufacture, Delivery and Pharmacokinetics for Optimizing Drug Adherence, Normalizing Breast Cancer Metabolism to Prevent Progression and Recurrence (PQ21), G2795275917, G3083819904, Chronic HIV Infection and Aging in NeuroAIDS (CHAIN) Center, G382641700, G4119534169, G419881185, G42916606, Nanomedicine and NeuroAIDS, G4501827968, Griffin: Venous Thromboembolism and mass spectrometry-based untargeted metabolomi, G498139845, G5850682332, G5959837446, SMART HAND, Adult Stem Cells for Therapy of Visual Disorders, METABOLOMIC IMAGING OF EARLY BREAST CANCER BRAIN METASTASIS TO IDENTIFY TARGETS FOR PREVENTION, Proteomic Strategies for AIDS and Drug Abuse - HIV and METH CNS Synergy, MicroRNAs for therapy of visual disorders, G71690937, G7354628648, Vascular mechanisms regulating breast cancer brain metastasis, G8632625050, Integrins and Ocular Angiogenesis, G9000346257, G969889393
OpenAlex ID: W2114431541