Work

The recombination rate is not increased when retroviral RNA is missing an encapsidation sequence

Year: 1996

Type: article

Abstract: Retroviruses, as a result of the presence of two identical RNA molecules in their virions, recombine at a high rate. When nonhomologous RNA is present in the dimer RNA molecules, nonhomologous recombination occurs, although the rate is only 0.1% of the rate of homologous recombination. We developed a protocol to study transduction of cellular proto-oncogenes in a single cycle of retrovirus replication. The psi sequences is a cis required sequence for packaging viral RNA into viral particles. To test if the rate of nonhomologous recombination increases about 1,000 times when the psi sequence is deleted, as reported by other, we modified vectors we used previously (J. Zhang and H. M. Temin, Science 259:234-238, 1993). Our results indicated that the recombination rate did not undergo the increase of about 1,000 times when the psi sequence of a chimeric RNA was deleted.

Source: Journal of Virology

DOI: 10.1128/jvi.70.3.2019-2021.1996

Authors: J Zhang, H M Temin

Institutions: University of Kentucky, Markey Cancer Center

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute

Awards: G1747792963, G222495705, CANCER CENTER SUPPORT, Molecular Biology and Genetics of Human Tumor Viruses