Work

The absence of effect of gid or mioC transcription on the initiation of chromosomal replication in Escherichia coli

Year: 1997

Type: article

Abstract: Despite the widely accepted view that transcription of gid and mioC is required for efficient initiation of cloned oriC, we show that these transcriptions have very little effect on initiation of chromosome replication at wild-type chromosomal oriC. Furthermore, neither gid nor mioC transcription is required in cells deficient in the histone-like proteins Fis or IHF. However, oriC that is sufficiently impaired for initiation by deletion of DnaA box R4 requires transcription of at least one of these genes. We conclude that transcription of mioC and especially gid is needed to activate oriC only under suboptimal conditions. We suggest that either the rifampicin-sensitive step of initiation is some other transcription occurring from promoter(s) within oriC, or the original inference of transcriptional activation derived from the rifampicin experiments is incorrect.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.94.23.12497

Authors: David B. Bates, Erik Boye, Tsuneaki Asai, Tokio Kogoma

Institutions: University of New Mexico, UNM Comprehensive Cancer Center, Harvard University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, Kreftforeningen, National Institutes of Health, National Institute of General Medical Sciences

Awards: Acquisition of an Autoradiogram Imaging System, G18718671, DNA REPLICATION IN E COLI--REGULATORY MUTANTS