Work

Inhibition of lung tumor growth in nude mice by siRNACD31 targeting PECAM-1

Year: 2014

Type: article

Abstract: Small interfering RNA (siRNA) provides a promising therapeutic approach in the silencing of disease‑causing genes. In the present study, the use of 2'‑O‑methyl‑modified siRNA‑cluster of differentiation 31 (siRNACD31), with cationic liposome RNA interference (RNAi)‑mate as a carrier, effectively silenced the platelet endothelial cell molecule 1 (PECAM‑1) gene of murine hemangioendothelioma cells in vitro. In vivo, 2'‑O‑methyl‑modified siRNACD31 carried by RNAi‑mate was successfully delivered, targeting the PECAM‑1 gene in the vasculature of nude mouse lung carcinoma xenografts. The growth of the lung carcinoma xenografts was inhibited by the 2'‑O‑methyl‑modified siRNACD31 and RNAi‑mate complexes, and the expression of the PECAM‑1 protein was downregulated, with a simultaneous decrease in vascular endothelial growth factor (VEGF) protein in the lung carcinoma xenografts. 2'‑O‑methyl‑modified siRNACD31‑RNAi‑mate complexes may provide a potential therapeutic strategy in lung carcinoma treatment. The effect of PECAM‑1 on VEGF expression may possibly be attributed to the function of PECAM‑1 signal transduction.

Source: Oncology Letters

DOI: 10.3892/ol.2014.2091