Work

Activation of the Pseudomonas aeruginosa Type III Secretion System Requires an Intact Pyruvate Dehydrogenase aceAB Operon

Year: 2002

Type: article

Abstract: Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.

Source: Infection and Immunity

DOI: 10.1128/iai.70.7.3973-3977.2002

Authors: Denis Dacheux, Olivier Epaulard, Arjan de Groot, Benoit Guery, Rozen Leberre, Ina Attree, Benoit Polack, Bertrand Toussaint

Institutions: Centre National de la Recherche Scientifique, Université de Bordeaux, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Centre Hospitalier Universitaire de Grenoble, CEA Grenoble, Centre Hospitalier de Tourcoing

Language: English

Topic: Bacterial biofilms and quorum sensing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2115634065

FWCI: 2.486

Cites: 20

Cited by: 104

Related to: 10