Work

Multi-color miniature dual-axis confocal microscope for point-of-care pathology

Year: 2012

Type: article

Abstract: We present a miniature microelectromechanical systems-based dual-axis confocal microscope capable of spatially coregistered fluorescence and reflectance imaging at multiple wavelengths. This device has a 10 mm diameter scan head with a 2 mm diameter tip for convenient use during surgery to guide tumor resection. The microscope has an adjustable focal depth of 20-200 micrometers and is capable of imaging with an axial resolution of 9 micrometers and in-plane resolution of 4 micrometers over a field of view of 450×450 micrometers. Simultaneous two-color imaging of individual optical sections is achieved by using a pair of grating-prism assemblies to compensate for chromatic dispersion in the 2 mm diameter gradient index relay lens at the distal tip of the device. Experimental measurements of the axial response of the microscope, as well as two-color images of a reflective bar target and fresh mouse brain tissues, demonstrate the performance of our device and its potential for multicolor in vivo optical sectioning microscopy.

Source: Optics Letters

DOI: 10.1364/ol.37.002430

Authors: Steven Y. Leigh, Jonathan T. C. Liu

Institution: Stony Brook University

Language: English

Topic: Advanced optical system design

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of Biomedical Imaging and Bioengineering

Awards: G1462008395, G1912377827, Dual-axis Confocal Microscope for Image-guided Resection of Medulloblastoma