A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
Year: 2011
Type: article
Abstract: Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
Source: Genome biology
Authors: Sheila Fisher, Andrew Barry, Justin Abreu, Brian Minie, Jillian Nolan, Toni M Delorey, Geneva Young, Timothy J Fennell, Alexander Allen, Lauren Ambrogio, Aaron M Berlin, Brendan Blumenstiel, Kristian Cibulskis, Dennis Friedrich, Ryan Johnson, Frank Juhn, Brian Reilly, Ramy Shammas, John Stalker, Sean M Sykes, Jon Thompson, John Walsh, Andrew Zimmer, Zac Zwirko, Stacey Gabriel, Robert Nicol, Chad Nusbaum
Institutions: Broad Institute, Foundation Medicine (United States)
Language: English
Topic: Genomics and Rare Diseases
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,490
OpenAlex ID: W2115875921