Work

Measuring the accuracy of genome-size multiple alignments

Year: 2007

Type: article

Abstract: Whole-genome alignments are invaluable for comparative genomics. Before doing any comparative analysis on a region of interest, one must have confidence in that region's alignment. We provide a methodology to measure the accuracy of arbitrary regions of these alignments, and apply it to the UCSC Genome Browser's 17-vertebrate alignment. We identify 9.7% (21 Mbp) of the human chromosome 1 alignment as suspiciously aligned. We present independent evidence that many of these suspicious regions represent misalignments.

Source: Genome biology

DOI: 10.1186/gb-2007-8-6-r124

Authors: Amol Prakash, Martin Tompa

Institution: University of Washington

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $5,490

Funders: National Science Foundation, National Institutes of Health, National Human Genome Research Institute, National Institute of General Medical Sciences