Work

Measurement of Small Quantities of Insulin-like Activity Using Rat Adipose Tissue. I. a Proposed Procedure*

Year: 1960

Type: article

Abstract: In the absence of sufficiently sensitive methods for the chemical determination of insulin, attempts to measure insulin in biological fluids have been almost entirely limited to bioassay procedures and, more recently, to immunological techniques. The bioassay procedures used have been based on the hypoglycemic effect of insulin in animals whose sensitivity was increased by endocrine manipula-tion (1-3) or on the effects of insulin on isolated tissue, in particular the mammary gland of lactat-ing rats (4), and the diaphragm of rats (5-8) or mice (9). The largest body of information cur-rently available concerns procedures based upon the effect of insulin on glucose uptake by rat diaphragm muscle (10).

Source: Journal of Clinical Investigation

DOI: 10.1172/jci104168

Authors: Albert E. Renold, Donald B. Martin, Yves M. Dagenais, Jurgen Steinke, Rita J. Nickerson, Mindel C. Sheps

Institution: Harvard University

Language: English

Topic: Pancreatic function and diabetes

Subfield: Endocrinology, Diabetes and Metabolism

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: American Diabetes Association, Eli Lilly and Company, U.S. Public Health Service