Work

Spider silk-bone sialoprotein fusion proteins for bone tissue engineering

Year: 2011

Type: article

Abstract: The remarkable mechanical characteristics of the spider silk protein major ampullate spidroin protein suggest this polymer as a promising biomaterial to consider for the fabrication of scaffolds for bone regeneration. Herein, a new functionalized spider silk-bone sialoprotein fusion protein was designed, cloned, expressed, purified and the osteogenic activity studied. Bone sialoprotein (BSP) is a multi-domain protein with the ability to induce cell attachment and differentiation and the deposition of calcium phosphates (CaP). Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) was used to assess the secondary structure of the fusion protein. In vitromineralization studies demonstrated that this new fusion protein with BSP retained the ability to induce the deposition of CaP. Studies in vitro indicated that human mesenchymal stem cells had significant improvement towards osteogenic outcomes when cultivated in the presence of the new fusion proteinvs. silk alone. The present work demonstrates the potential of this new fusion protein for future applications in bone regeneration.

Source: Soft Matter

DOI: 10.1039/c1sm05024a

Authors: Sílvia Gomes, Isabel B. Leonor, João F. Mano, Rui L. Reis, David L. Kaplan

Institutions: Tufts University, Institute for Biotechnology and Bioengineering, 3B's Research Group, University of Minho

Language: English

Topic: Silk-based biomaterials and applications

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2116684044

FWCI: 1.977

Cites: 58

Cited by: 50

Related to: 10