Work

Galectin-1 is a stromal cell ligand of the pre-B cell receptor (BCR) implicated in synapse formation between pre-B and stromal cells and in pre-BCR triggering

Year: 2002

Type: article

Abstract: Although preB cell-receptor (pre-BCR) formation and cell-surface expression is essential for B cell development, pre-BCR generation of signal transduction remains elusive. Here, we report that recombinant pre-BCRs and the surrogate light chain bind specifically to the bone marrow stromal cell galectin-1 (GAL1), an S-type lectin. The surrogate light chain/GAL1 association is a direct protein-protein interaction (K(a) = 2 x 10(6) M(-1)), and the NH(2) extra loop of lambda-like is the major binding element. Pre-BCR binding to stromal cells depends upon GAL1 anchoring to glycosylated counter-receptors, and these complexes completely relocalize to form a synapse at the contact zone between preB and stromal cells. This immune developmental synapse is accompanied by the initiation of intracellular tyrosine kinase activity and signal transduction from the pre-BCR.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.202323999

Authors: Laurent Gauthier, Benjamin Rossi, Florence Roux, Elise Termine, Claudine Schiff

Institutions: Centre National de la Recherche Scientifique, Inserm, Centre d’Immunologie de Marseille-Luminy

Language: English

Topic: Galectins and Cancer Biology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique