Work

Light‐Activated Gene Editing with a Photocaged Zinc‐Finger Nuclease

Year: 2011

Type: article

Abstract: Light, zinc-finger nuclease, action: Using unnatural amino acid mutagenesis, a light-activated zinc-finger nuclease enzyme was engineered through incorporation of a photocaged tyrosine residue at a DNA–protein interface. The caged zinc-finger nuclease was completely inactive until irradiated with UV light, thus enabling photochemical control of sequence specific gene editing in mammalian cells (see picture).

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201101157

Authors: Chungjung Chou, Alexander Deiters

Institution: North Carolina State University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: Switchable Systems for Spatio-Temporal Control of Gene Expression in Zebrafish, G6103147385